← The elements of bacteriological technique
Chapter 90
Tables for Preparing Dilutions
TABLE I
Using 10 % stock solution
First dilution + Diluent TABLE II
Using 1% stock solution
Second dilution + Diluent
1: 10 = 1 c.c. + 0 c.c. 1: 100 = 1 c.c. + 0 c.c.
1: 15 = 1 c.c. + 0.5 c.c. 1: 110 = 1 c.c. + 0.1 c.c.
1: 20 = 1 c.c. + 1.0 c.c. 1: 120 = 1 c.c. + 0.2 c.c.
1: 25 = 1 c.c. + 1.5 c.c. [1: 125 = 1 c.c. + 0.25 c.c.]
1: 30 = 1 c.c. + 2.0 c.c. 1: 130 = 1 c.c. + 0.3 c.c.
1: 35 = 1 c.c. + 2.5 c.c. 1: 140 = 1 c.c. + 0.4 c.c.
1: 40 = 1 c.c. + 3.0 c.c. 1: 150 = 1 c.c. + 0.5 c.c.
1: 45 = 1 c.c. + 3.5 c.c. 1: 160 = 1 c.c. + 0.6 c.c.
1: 50 = 1 c.c. + 4.0 c.c. 1: 170 = 1 c.c. + 0.7 c.c.
1: 55 = 1 c.c. + 4.5 c.c. [1: 175 = 1 c.c. + 0.75 c.c.]
1: 60 = 1 c.c. + 5.0 c.c. 1: 180 = 1 c.c. + 0.8 c.c.
1: 65 = 1 c.c. + 5.5 c.c. 1: 190 = 1 c.c. + 0.9 c.c.
1: 70 = 1 c.c. + 6.0 c.c. 1: 200 = 1 c.c. + 1.0 c.c.
1: 75 = 1 c.c. + 6.5 c.c. ————————————————-
1: 80 = 1 c.c. + 7.0 c.c. 1: 200 = 1 c.c. + 1.0 c.c.
——————————————— 1: 225 = 1 c.c. + 1.25 c.c.
1: 80 = 1 c.c. + 7.0 c.c. 1: 250 = 1 c.c. + 1.5 c.c.
1: 90 = 1 c.c. + 8.0 c.c. 1: 275 = 1 c.c. + 1.75 c.c.
1: 100 = 1 c.c. + 9.00 c.c. 1: 300 = 1 c.c. + 2.0 c.c.
1: 110 = 1 c.c. + 10.0 c.c. 1: 325 = 1 c.c. + 2.25 c.c.
1: 120 = 1 c.c. + 11.0 c.c. 1: 350 = 1 c.c. + 2.5 c.c.
[1: 125 = 1 c.c. + 11.5 c.c.] 1: 375 = 1 c.c. + 2.75 c.c.
1: 130 = 1 c.c. + 12.0 c.c. 1: 400 = 1 c.c. + 3.0 c.c.
1: 140 = 1 c.c. + 13.0 c.c. ————————————————-
1: 150 = 1 c.c. + 14.0 c.c. 1: 400 = 1 c.c. + 3.0 c.c.
1: 160 = 1 c.c. + 15.0 c.c. 1: 450 = 1 c.c. + 3.5 c.c.
1: 170 = 1 c.c. + 16.0 c.c. 1: 500 = 1 c.c. + 4.0 c.c.
[1: 175 = 1 c.c. +-16.5 c.c.] ————————————————-
1: 180 = 1 c.c. + 17.0 c.c. 1: 500 = 1 c.c. + 4.0 c.c.
1: 190 = 1 c.c. + 18.0 c.c. 1: 600 = 1 c.c. + 5.0 c.c.
1: 200 = 1 c.c. + 19.0 c.c. 1: 700 = 1 c.c. + 6.0 c.c.
———————— —————— [1: 750 = 1 c.c. + 6.5 c.c.]
1: 200 = 1 c.c. + 19.0 c.c. 1: 800 = 1 c.c. + 7.0 c.c.
1: 225 = 1 c.c. + 21.5 c.c. 1: 900 = 1 c.c. + 8.0 c.c.
1: 250 = 1 c.c. + 24.0 c.c. 1: 1000 = 1 c.c. + 9.0 c.c.
1: 275 = 1 c.c. + 26.5 c.c. ————————————————
1: 300 = 1 c.c. + 29.0 c.c. 1: 1000 = 1 c.c. + 9.0 c.c.
1: 325 = 1 c.c. +-31.5 c.c. 1: 2000 = 1 c.c. + 19.0 c.c.
1: 350 = 1 c.c. + 34.0 c.c. 1: 3000 = 1 c.c. + 29.0 c.c.
1: 375 = 1 c.c. + 36.5 c.c. 1: 4000 = 1 c.c. + 39.0 c.c.
1: 400 = 1 c.c. + 39.0 c.c. 1: 5000 = 1 c.c. + 49.0 c.c.
1: 400 = 1 c.c. + 39.0 c.c.
1: 450 = 1 c.c. + 44.5 c.c.
1: 500 = 1 c.c. + 49.0 c.c.
TABLE III
Using 0.1% stock solution
Third dilution + Diluent TABLE IV
Using 0.01% stock solution
Fourth Dilution + Diluent
1: 1000 = 1 c.c. + 0 c.c. 1: 10,000 = 1 c.c. + 0 c.c.
1: 1250 = 1 c.c. + 0.25 c.c. 1: 15,000 = 1 c.c. + 0.5 c.c.
1: 1500 = 1 c.c. + 0.5 c.c. 1: 20,000 = 1 c.c. + 1.0 c.c.
1: 1750 = 1 c.c. + 0.75 c.c. 1: 25,000 = 1 c.c. + 1.5 c.c.
1: 2000 = 1 c.c. + 1.0 c.c. 1: 30,000 = 1 c.c. + 2.0 c.c.
1: 2250 = 1 c.c. + 1.25 c.c. 1: 35,000 = 1 c.c. + 2.5 c.c.
1: 2500 = 1 c.c. + 1.5 c.c. 1: 40,000 = 1 c.c. + 3.0 c.c.
1: 2750 = 1 c.c. + 1.75 c.c. 1: 45,000 = 1 c.c. + 3.5 c.c.
1: 3000 = 1 c.c. + 2.0 c.c. 1: 50,000 = 1 c.c. + 4.0 c.c.
1: 3250 = 1 c.c. + 2.25 c.c. 1: 55,000 = 1 c.c. + 4.5 c.c.
1: 3500 = 1 c.c. + 2.5 c.c. 1: 60,000 = 1 c.c. + 5.0 c.c.
1: 3750 = 1 c.c. + 2.75 c.c. 1: 65,000 = 1 c.c. + 5.5 c.c.
1: 4000 = 1 c.c. + 3.0 c.c. 1: 70,000 = 1 c.c. + 6.0 c.c.
1: 4250 = 1 c.c. + 3.25 c.c. 1: 75,000 = 1 c.c. + 6.5 c.c.
1: 4500 = 1 c.c. + 3.5 c.c. 1: 80,000 = 1 c.c. + 7.0 c.c.
1: 4750 = 1 c.c. + 3.75 c.c. 1: 85,000 = 1 c.c. + 7.5 c.c.
1: 5000 = 1 c.c. + 4.0 c.c. 1: 90,000 = 1 c.c. + 8.0 c.c.
1: 5000 = 1 c.c. + 4.0 c.c. 1: 100,000 = 1 c.c. + 9.0 c.c.
1: 6000 = 1 c.c. + 5.0 c.c. ——————————
1: 7000 = 1 c.c. + 6.0 c.c. 1: 100,000 = 0.1 c.c. + 0.9 c.c.
[1: 7500 = 1 c.c. + 6.5 c.c.] 1: 200,000 = 0.1 c.c. + 1.9 c.c.
1: 8000 = 1 c.c. + 7.0 c.c. [1: 250,000 = 0.1 c.c. + 2.4 c.c.]
1: 9000 = 1 c.c. + 8.0 c.c. 1: 300,000 = 0.1 c.c. + 2.9 c.c.
1: 10,000 = 1 c.c. + 9.0 c.c. 1: 400,000 = 0.1 c.c. + 3.9 c.c.
——————————————— 1: 500,000 = 0.1 c.c. + 4.9 c.c.
1: 10,000 = 1 c.c. + 9.0 c.c. —————————————————-
1: 15,000 = 1 c.c. + 14.0 c.c. 1: 500,000 = 0.1 c.c. + 4.9 c.c.
1: 20,000 = 1 c.c. + 19.0 c.c. 1: 600,000 = 0.1 c.c. + 5.9 c.c.
1: 25,000 = 1 c.c. + 24.0 c.c. 1: 700,000 = 0.1 c.c. + 6.9 c.c.
1: 30,000 = 1 c.c. + 29.0 c.c. [1: 750,000 = 0.1 c.c. + 7.4 c.c.]
———————————————— 1: 800,000 = 0.1 c.c. + 7.9 c.c.
1: 900,000 = 0.1 c.c. + 8.9 c.c.
1:1,000,000 = 0.1 c.c. + 9.9 c.c.