Table 27

Organism employed, B. Coli Communis.
Culture Medium, Nutrient Agar (+10). Age, 24 hrs. Temp. of Incubation, 37°C.

Quantities used { Culture} Emulsion 0.1 c.c. + 5 c.c. Germicide.

{ Emulsion }

Room Temperature during Experiments, 17°C.

Germicide Strength Time of exposure Incubation

2-1/2 5 10 15 20 25 30 35 Time Temp.

1 Germicide-x 4% — — — — — — — — 7 days. 37°C.

2 Germicide-x 3% 48 — — — — — — — 7 days. 37°C.

3 Germicide-x 2% 24 24 24 24 48 72 ... ... 7 days. 37°C.

4 Germicide-x 1% 24 24 24 24 72 24 72 ... 7 days. 37°C.

5 Germicide-x 0.5% 24 24 24 24 24 24 24 24 24 hours. 37°C.

1 Phenol 1.10% — — — — — — — — 7 days. 37°C.

2 Phenol 1.00% 24 ... ... ... ... ... ... ... 7 days. 37°C.

3 Phenol 0.75% 24 24 24 24 48 ... ... ... 7 days. 37°C.

4 Phenol 0.70% 24 24 24 24 24 72 ... ... 7 days. 37°C.

5 Phenol 0.65% 24 24 24 24 24 48 24 24 2 days. 37°C.

((1.10/4.00) + (0.7/2.0)) 0.27 + 0.35 .62

Phenol Coefficient = ———————————— = —————— = —— = 0.31

2 2 2