Scheme of Study

Designation:

Originally isolated by (observer's name) in (date), from (source of organism).

1. Cultural Characters.—(Vide Macroscopical Examination of Cultivation, page 261.)

Gelatine plates, }

Gelatine streak, } at 20°C.

Gelatine stab, }

Gelatine shake, }

Agar plates, }

Agar streak or smear, }

Agar stab, }

Inspissated blood-serum, } at 20° C. and 37°C.

Bouillon, }

Litmus milk, }

Potato, }

Special media for the purpose of demonstrating characteristic appearances.

2. Morphology.—(Vide Microscopical Examination of Cultivations, page 272.)

Vegetative forms:
Shape.
Size.
Motility.
Flagella (if present).
Capsule (if present).
Involution forms.
Pleomorphism (if observed).
Sporing forms (if observed). Of which class?
Staining reactions.

3. Chemical Products of Growth.—(Vide Chemical Examination of Cultivations, page 276.)

Chromogenesis.
Photogenesis.
Enzyme formation.
Fermentation of carbohydrates:
Acid formation.
Alkali formation.
Indol formation.
Phenol formation.
Reducing and oxidising substances.
Gas formation.

4. Biology.—(Vide Physical Examination of Cultures, page 295.)

Atmosphere.
Temperature.

Reaction of nutrient media.
Resistance to lethal agents:
Physical:
Desiccation.
Light.
Colours.
Chemical germicides.
Vitality.

5. Pathogenicity:

Susceptible animals, subsequently arranged in order of susceptibility.
Immune animals.
Experimental inoculation, symptoms of disease.
Post-mortem appearances.
Virulence:
Length of time maintained.
Optimum medium?
Minimal lethal dose.
Exaltation and attenuation of virulence?
Toxin formation.